View post tag: International Six Defence teams drawn from Navy, Army, Air Force and the Australian Public Service competed yesterday in a ‘tug of war’ competition to celebrate International Women’s Day.The event was held at the Department of Defence headquarters at Russell Offices, and featured six teams: Navy, Army, Air Force, Royal Military College – Duntroon, the Australian Defence Force Academy and Defence People Group.Event organiser Group Captain Dee Gibbon said the event was a light-hearted tribute to military fitness with teams encouraged to dress in costume. “There is healthy competition between the three services. Each team had equal numbers of men and women and showcased how everyone in Defence, whether military or civilian, works together towards common goals,” said GPCAPT Gibbon.“Today was a lot of fun but also highlights the importance Defence places on days like International Women’s Day and the teamwork inherent within our working environment.”Two prizes were presented after the event: one to the winners, and one to the best-dressed team. The overall winner was the Royal Military College team, and the best-dressed prize awarded to a mixed team of public servants and military members representing the Defence People Group. Chief of the Defence Force, General David Hurley, presented the prizes to the teams. “This was a great event to mark International Women’s Day. I congratulate the winners on their skill and participation, and for making time to recognise this great cause,” General Hurley said.[mappress]Naval Today Staff, March 8, 2013; Image: Australian Navy Australian Defence Teams Mark International Women’s Day View post tag: teams View post tag: Navy Back to overview,Home naval-today Australian Defence Teams Mark International Women’s Day View post tag: News by topic Share this article View post tag: day View post tag: Defence View post tag: mark View post tag: Australian View post tag: Australia Training & Education March 8, 2013 View post tag: Women View post tag: Naval
The notion that police can identify a suspect based on the tiniest drop of blood or trace of tissue has long been a staple of TV dramas, but scientists at Harvard have taken the idea a step further. Using just a single human cell, they can reproduce an individual’s entire genome.As described in a Dec. 21 paper in Science, a team of researchers, led by Xiaoliang Sunney Xie, the Mallinckrodt Professor of Chemistry and Chemical Biology, and made up of postdoctoral fellow Chenghang Zong, graduate student Alec Chapman, and former graduate student Sijia Lu, developed a method — dubbed MALBAC, short for Multiple Annealing and Looping-based Amplification Cycles — that requires just one cell to reproduce an entire DNA molecule.More than three years in the making, the breakthrough technique offers the potential for early cancer treatment by allowing doctors to obtain a genetic “fingerprint” of a person’s cancer from circulating tumor cells. It also could lead to safer prenatal testing for a host of genetic diseases.“If you give us a single human cell, we report to you 93 percent of the genome that contains three billion base pairs, and if there is a single base mutation, we can identify it with 70 percent detectability, with no false positives detected,” Xie said. “This is a major development.”In a second paper, published simultaneously, researchers from Xie’s lab worked with scientists at Peking University in China to demonstrate MALBAC by sequencing 99 sperm cells from one individual and examining the paternal and maternal contribution to each cell’s genome.As its name suggests, Xie said, MALBAC is a type of DNA amplification that allows researchers to duplicate the single DNA molecule present in a cell many times so it can be analyzed in the lab.“While other methods of DNA amplification exist, most — like polymerase chain reaction (PCR) or multiple displacement amplification (MDA) — suffer from a specific problem,” Xie said. “Because they amplify exponentially, both have bias. They dramatically amplify some parts of the genome, but amplify others very little.”By comparison, he said, MALBAC relies on linear amplification, meaning it is able to minimize the sequence-dependent bias.Just as it does with other methods, the amplification process begins by splitting the DNA double helix into two single strands. Xie’s team then adds a random “primer” — tiny fragments of DNA — that binds in dozens of locations along each strand.To extend those primers, Xie’s team used a DNA polymerase, the same cellular “machine” that synthesizes DNA as cells divide. Using that machine, researchers are able to extend the primers from as few as seven bases to as many as 2,000. Upon heating, they break the elongated primers apart from the original DNA, yielding half products.When those half products are then amplified using the same primers, the two ends of the DNA combine, forming a loop that prevents it from being amplified again. The leftover half products and the original DNA are subject to another cycle of amplification. After five cycles of such linear pre-amplification, the full product is amplified by PCR to produce enough material for sequencing.Despite the high coverage, DNA polymerases do occasionally make errors, Xie explained. To ensure that the genome produced by MALBAC is accurate, researchers turned to a different technique.“Many diseases are associated with a single base mutation,” Xie said. “The challenge, however, is that finding one mutation in more than 3 billion base pairs is like looking for a needle in a haystack. Earlier techniques, like PCR or MDA, start with many cells, making the challenge even greater; a single mutation simply gets lost in the process. MALBAC, however, starts with a single cell, so it is easier to identify those mutations when they happen.”To ensure MALBAC’s accuracy, Xie’s team simply let the original cell divide.While the polymerase that researchers use to build the DNA sequence is highly accurate, only making one mistake per 10,000 bases, letting the cell divide gives researchers a chance to double check its work.“The chances of the same mistake being made at the same base position are about one in 100 million,” Xie said. “If we let the cells divide again, and sequence three cells, the chances go up to one in 10 billion, less than the number of bases in the entire DNA molecule, so we can remove all the false positives.“Getting that level of accuracy is very important, because if a doctor tells a patient that he detects a mutation, he doesn’t want to be wrong,” he continued. “When we use MALBAC, if a mutation appears in two or three related cells, we know it must be a real mutation.”As a demonstration of MALBAC’s power, Xie and his team monitored the mutations that arose in a single cancer cell as it divided over 20 generations, and uncovered as many as 50 newly acquired mutations.“This is the first time the mutation rate of a human cell has been measured directly,” Xie said. “Because we can now see the unique, newly acquired bases, we can study the dynamics of the genome in a way that was not possible before.”
Teens tackle question of freedom in America Related Coed Hasty Pudding makes its debut A revolutionary musical Project lets students write and perform ‘Freedom Acts’ for the A.R.T. stage Women perform alongside male counterparts for first time in group’s 171-year history They are the very models of two modern major influencers.Gilbert and Sullivan are still very much part of the scene, nearly 150 years after their first production. Their 14 Victorian-era comic operas, wildly popular in their time, paved the way for the development of British and American musicals. And today their works remain among the most beloved, staged, and referenced pieces of musical theater in pop culture — from “Hamilton” to “The Simpsons,” “Family Guy” to “The West Wing” to Lizzo.This Friday, that high-voltage, fanciful, satiric tradition continues at Harvard as the Harvard-Radcliffe Gilbert and Sullivan Players set sail on a nine-show run of “H.M.S. Pinafore” at the Agassiz Theatre.The love-conquers-all comic opera about a captain’s daughter and a lowly sailor was the fourth collaboration of dramatist W.S. Gilbert and composer Arthur Sullivan and arguably their most famous work, running for a near-record 571 performances after its premiere in London in 1878.“You just can’t listen to Gilbert and Sullivan without being entirely entranced,” said Ross Simmons ’21, the troupe’s historian. “The music just lifts your soul out of your chest” and the dialogue “contains great poetry.”Performing Gilbert and Sullivan on campus since 1956, Harvard’s resident company has become one of the leading troupes in New England dedicated to the work of the duo, consistently staging among the most well-attended and lavishly produced shows on campus.As with all the group’s performances, members are responsible for all aspects of production, from the creative to the technical. They select the show, set the budget, cast the actors, do the lighting, sets, and costumes, direct, and choreograph. The effort is massive and takes months of logistical work and weeks of rehearsal.,On campus, the company is known for its welcoming community and camaraderie. Anyone can audition for roles, and students who wish to join need only reach out, even if they’re not undergraduates, or even Harvard students. As a result, the members of the company often include Harvard graduate students, undergraduates from other universities, and Cambridge and Boston residents. This year, for instance, the music director, the male lead, and a number of orchestra members are from Tufts University.“I’ve done a lot of theater, and I’ve never seen a group of people come together in a way that [the Harvard-Radcliffe players] do,” said Mary Reynolds, the music director and a junior at Tufts.The company also sets no requirement for previous theater experience and encourages members to seek out roles that will push them out of their comfort zones.That’s the really beautiful thing about the Harvard G&S players, said Sabrina Richert ’20, the troupe’s president. “Everyone has a chance to try something new and learn together and from each other.” There’s a system “built in of people that can support and help you,” added Jamie Ostmann ’21, the group’s costume designer, who is taking the stage for the first time as part of the female ensemble. She noted the mentoring and encouragement she’s received from stage veterans running up to the premiere.The community extends beyond the rehearsals and performances. Members past and present become familiar through a number of informal and formal social events the troupe’s leadership organizes, including a Victorian Ball in the spring.With this type of structure in place, “you get a community where people keep coming back,” stage manager Ava Hampton ’21 said.Made up of about 60 members, the company ranges from Gilbert and Sullivan diehards to those getting their first taste.,Janiah Lockett ’20 was one of the uninitiated. Until a few years she didn’t know anything about Gilbert and Sullivan. Now, she is well-versed in their history and cultural impact, and this show marks her fourth production with the troupe — her first in the director’s chair.“After my first production, I just fell in love with it,” she said. The wordplay. The wit. The music. She loved all of it. “I just thought, ‘How are not more people doing this? This is so fun it’s incredible.’”With opening night days away, she feels the cast is in good shape after watching them bring the whimsy to life at a recent rehearsal. Yet while she is excited about the premiere, she admits it will be bittersweet, especially for the group’s seniors, like her, because it marks the last time they’ll work on a Gilbert and Sullivan operetta as undergraduates.Next semester, the group plans to take a break from the work of their namesake comic opera legends, staging Alan Jay Lerner and Frederick Loewe’s “My Fair Lady.”The company is doing so in an effort to expand its audience, said Richert. In particular, it wants to reach first-years who may consider the operettas dated. They hope new students will get involved and stay involved by attending or helping produce future G&S-focused shows, as Lockett and others have.“We think that this will be a good opportunity for recruiting and getting more people involved in the community on campus,” said Richert, who noted the break with canon will happen every two to four years. “We feel that expanding our repertoire a little bit will help bring new people into our organization and generate some more enthusiasm.”Ostmann agrees. The group is “like this little hidden secret of the Harvard community,” she said — and it’s OK to let everyone else in on it, too.The Harvard-Radcliffe Gilbert and Sullivan Players will perform “H.M.S. Pinafore” at the Agassiz Theatre on Nov. 8‒10, and 14‒17. Tickets are available. ‘We Live in Cairo,’ about the 2011 Egyptian uprising, premieres at A.R.T.
51 Mandalay St, Fig Tree Pocket. 51 Mandalay St, Fig Tree Pocket. 51 Mandalay St, Fig Tree Pocket.NGU Realestate — East Brisbane selling agent Emil Juresic said the generous size of the home, combined with its proximity to Brisbane’s CBD, made it an ideal purchase for a family seeking privacy, convenience and a touch of luxury.The property is close to parks, Lone Pine Koala Sanctuary and several schools. Brett Shepherd and his wife Julie are ready to downsize from their beautiful seven-bedroom, three-bathroom home at Fig Tree Pocket. A builder with AusHomes, Mr Shepherd moved into the house he built in 2009.He said their children had grown up and left home.“It’s just too big for us,” Mr Shepherd said.“It’s an absolutely gorgeous home, it’s north-facing up the river.“It’s the most peaceful, tranquil place to live in.” 51 Mandalay St, Fig Tree Pocket.Mr Shepherd said he enjoyed waking up every morning to the birds singing outside.He said the home had every mod con known to mankind including Cbus lighting, two balconies, a cinema, study, a salt water swimming pool and fountains, and security intercom.More from newsDigital inspection tool proves a property boon for REA website3 Apr 2020The Camira homestead where kids roamed free28 May 2019Mr Shepherd said his favourite part of the house was the outside area.“I love sitting outside, overlooking the river and the pool,” he said.“This home has water views from every aspect.” 51 Mandalay St, Fig Tree Pocket.The Shepherd’s have had many memories living in the home including birthdays and anniversaries.“Many memories are made when you raise a family,” he said.“I’d be happy to live here forever however this home needs another family to move into it.”
Batesville, IN—The Batesville Area Chamber of Commerce is seeking nominations for its annual awards. The awards include the Distinguished Service, Educator of the Year, Organization of the Year, and Volunteer of the Year. The nomination deadline is December the 31st and can be turned in to the Batesville Chamber office.
Every two weeks, Mike Mandell gives his thoughts on the sports scene in Ellsworth, Hancock County and beyond.If you spend year after year as an ardent follower of any sports team, you’ll eventually encounter a group that’s different from the rest. Maybe it has a once-in-a-lifetime coach at the helm. Perhaps it has a collection of players who complement each other well or have endured particular hardships together. In any case, there are certain teams we remember forever.In the world of high school sports, those teams pass us by in an instant. With athletes coming and going in just four short years, squads that seem destined for greatness have small windows of opportunity to succeed. Expectations for those groups are high, and cities and towns that are passionate about their local teams can put an enormous amount of pressure on young athletes’ shoulders.That’s what has made the successes of this year’s George Stevens Academy and Mount Desert Island boys’ basketball teams so remarkable. Every team that has what it takes expects to be the last one standing, but that doesn’t always happen. Come tournament time, a brief stretch of poor play is all it takes to send your team packing. GSA and MDI avoided that fate.GSA head coach Dwayne Carter knew his team had a chance to be special before the 2016-17 season started. After all, the Eagles were returning four of five starters from a team that won the Class C title. They had every piece in place to do it again this time around, and expectations in Blue Hill were through the roof.This is placeholder textThis is placeholder textIn the regular season, the Eagles couldn’t be stopped. They beat opponents by 30 and 40 points on a nightly basis and were only challenged in road games against Ellsworth and Lee Academy. GSA was the best show around, and everyone from Bucksport to East Sullivan knew it.Yet there were times in the tournament when the outlook didn’t seem so rosy. GSA couldn’t pull away from Fort Fairfield until the very end of the Class C North final, and with Winthrop ahead by six with two minutes left Saturday, the Eagles’ undefeated season was in dire peril. It wasn’t until the last second — literally — that they could breathe a sigh relief as state champions for the second year in a row.MDI was in a similar situation. The Trojans had nearly everyone back from a team that finished 15-5 last year, and they entered the season as one of the favorites in Class B. Groups with the depth and athleticism this MDI senior class had are exceptionally rare, and some sensed the season to come would be the team’s best shot at winning the Gold Ball.Despite a 13-0 start, that season would soon show signs of unraveling. As January turned to February, the Trojans’ early exploits had been forgotten amidst a stretch in which they lost three of four games. With the tournament just around the corner, it was the worst time to hit a lull.Instead of fizzling out, MDI bounced back stronger than ever. The Trojans used stout defense to methodically dispatch Belfast, Central and Orono for their first regional championship since 1995, and they did the same in a dominant win against Wells that saw them become the first MDI boys’ team in history to claim the state title.For every group that comes together as GSA and MDI did, there are far more that don’t. Only 10 of the 250-plus boys’ and girls’ teams from around the state can bring home titles in a given year, and there are always going to be championship-worthy teams that fall short of winning it all. Such an outcome is unavoidable.The teams that do win always have something more to them, though. Sure, GSA and MDI were experienced and talented groups that played well together, but such descriptions can fit any number of teams. Even with the right coaches and the right schemes, you have to have the right among of luck on your side. The teams that ride that luck to glory are usually the ones that have done everything else the right way.Hancock County was fortunate to have teams like GSA and MDI this season because they don’t come around often. Even when they do, they aren’t guaranteed to win championships — especially in a tournament that requires you to beat the best of the best every time you set foot on the court.That’s all the more reason to celebrate when local teams accomplish such a feat. The teams that achieve what so many seek can be described in one word: legendary. The 2016-17 GSA and MDI teams have proven themselves worthy of that label.GRAPHIC BY TIM SUELLENTROP Latest Posts Latest posts by Mike Mandell (see all) Hospice volunteers help families navigate grief and find hope – September 12, 2020 Bio Ellsworth runners compete in virtual Boston Marathon – September 16, 2020 Mike MandellMike Mandell is the sports editor at The Ellsworth American and Mount Desert Islander. He began working for The American in August 2016. You can reach him via email at [email protected] MPA approves golf, XC, field hockey, soccer; football, volleyball moved to spring – September 10, 2020
Defending champions UL are without Tony Kelly as they take-on Mary I in Belfast.Also this afternoon, LIT will play host to UCD while UUJ take-on Cork IT in Jordanstown and DIT travel to Dangan to face NUIG.Those matches will all throw-in at 2pm, while there’s also two games that will get underway at 7 o’clock as UCC travel to Maynooth and WIT go up against DCU in Carriganore.