Zach Randolph scored 21 points and snagged 12 rebounds to help the Memphis Grizzlies beat the Golden State Warriors 88-81 in overtime on Wednesday.Randolph was 9-of-22 shooting from the field and with his efforts kept the Grizzlies close in the third quarter. Marc Gasol had 18 points and 11 rebounds. Mike Conley started out rough missing 10 of his 18 shots before sinking a wide-open jumper from beyond the arc to give Memphis an 82-79 lead in overtime.”New coach, new offense … we just have to get used to it,” said Conley, who finished with 19 points, six rebounds and four assists. ”Now we’ve been able to figure it out.”Andrew Bogut had 12 points and 14 rebounds for Golden State. Klay Thompson netted 21 points without the help of Stephen Curry who was benched after suffering a concussion when he hit his head on the floor while diving for a loose ball in Monday’s game against Utah.Neither team scored in the final 2:02 of regulation and back-to-back buckets by Golden State’s David Lee and Klay Thompson evened the contest at 79-79 before OT.Conley then hit a 3-pointer to put Memphis in front and after Thompson was whistled for an offensive foul, Tayshaun Prince knocked down a left wing jumper to give Memphis an 84-79 cushion.Warriors’ Andrew Bogut then threw down Andre Iguodala’s alley-oop feed before Prince nailed a tough step-back jumper from the top of the key extending their lead to 86-81.Warriors head coach Mark Jackson wasn’t too pleased with his team’s efforts in the final minutes of the game.“We wanted to push the pace,” Jackson said. “That’s when then game changed when we started to walk it up the floor.”The win completed a four-game West Coast sweep for Memphis after the Grizzlies had dropped two of three at home.
Roberto Pereyra came to Watford back in 2016 from Juventus and it was a surprising move for a lot of people as he had a good position in his former team – but he wanted to play in the Premier League.Gino Pozzo, the current Watford owner, brought Pereyra to Udinese a couple of years ago and he played a crucial role also in his move to England as the player insisted that he persuaded him to join the Hornets.The former Juventus midfielder spoke about this transfer as he said, according to hertfordshiremercury.co.uk:“Gino was the determining factor.”Fiorentina owner: “Ribery played better than Ronaldo!” Andrew Smyth – September 14, 2019 Fiorentina owner Rocco Commisso was left gushing over Franck Ribery’s performance against Juventus, which he rates above that of even Cristiano Ronaldo’s.“I obviously knew him from the past at Udinese and knowing how he works. I know he believed in me and that I could trust him. Having Gino was one of my main factors.”“It was a very hard choice because Juventus were winning many things. We are talking about one of the greatest clubs in the world.”“Yes, this is the Premier League but Juventus, well, are Juventus. At the beginning, it was complicated – it was another country, there was this whole settling in. It was quite overwhelming.”
Juventus manager Massimiliano Allegri has hailed Cristiano Ronaldo as “extraordinary” ahead of their first leg against Real Madrid on TuesdayThe Portuguese star played an integral part in Real’s 4-1 win over Juventus in last season’s Champions League final at Cardiff by bagging a brace.Ronaldo has scored 36 goals in his 33 appearances in all competitions this season and Allegri hailed the forward’s transition into adapting towards the latter stages of his career.“We have to be very good as a unit [to stop him],” said Allegri, according to Football-Espana.Fiorentina owner: “Ribery played better than Ronaldo!” Andrew Smyth – September 14, 2019 Fiorentina owner Rocco Commisso was left gushing over Franck Ribery’s performance against Juventus, which he rates above that of even Cristiano Ronaldo’s.“I think we need to congratulate Ronaldo because for a player to be 30 or 31 and change his playing style completely is something extraordinary.”The Italian, who was recently awarded the Panchina d’Oro for a third time, believes that Gareth Bale more likely to start for Real than Isco.“Isco or Bale to start? You’d have to ask Zidane as I am the Juventus Coach and I am in no position to make a call or comment about it.“Honestly, I think Bale may be more likely but then maybe it will be Isco, I honestly do not know.”
Tony Cascarino believes the Egyptian player is frustrated right now at English Premier League club LiverpoolLiverpool won at home against French Ligue 1 side Paris Saint-Germain.And even though Egyptian footballer Mohamed Salah was constantly looking for a goal, he couldn’t get one.For former Chelsea player Tony Cascarino, Salah is having a difficult time right now in the English Premier League and the UEFA Champions League.“Mo Salah is having a difficult time, I think that’s clear,” Cascarino told talkSPORT.Virgil van Dijk praises Roberto Firmino after Liverpool’s win Andrew Smyth – September 14, 2019 Virgil van Dijk hailed team-mate Roberto Firmino after coming off the bench to inspire Liverpool to a 3-1 comeback win against Newcastle United.“He’s a really frustrated player at the moment, just watch his reaction after Liverpool go 3-2 up and win the game.”“You can clearly see his reaction after [Roberto] Firmino scores the winning goal, he throws a bottle to the ground with anger, you can clearly see that,” he added.“It’s been a tricky last few months for Mo Salah, but it’s going under the radar slightly because Liverpool has won every game.”“Harry Kane has been in the newspapers about his form, it’s all been Harry, Harry, Harry, and yet Mo’s level of performances are clearly nowhere near last year,” Cascarino continued.“You look at him winning all the Player of the Year awards but then getting injured in the Champions League final last season, it’s all gone backward from there.”
Liverpool strike duo Mohamed Salah and Sadio Mane are the three finalists for the 2018 CAF Player of the Year award along with Pierre-Emerick AubameyangLast year’s winner Salah will be looking to add another to his growing collection of individual accolades following a breakthrough debut campaign at Liverpool.The Egyptian superstar scored 44 goals in 52 games across all competitions last season and set a new Premier League record of 32 goals in a 38-game campaign.Salah’s team-mate Mane, who finished runner-up in 2017, also impressed at Anfield and scored in Liverpool’s Champions League final defeat to Real Madrid.The Senegal international, who has found the net eight times in 18 league games this term, has formed part of a devastating strike partnership with Salah and Roberto Firmino for the Reds.LONDON, ENGLAND – DECEMBER 22: Pierre-Emerick Aubameyang of Arsenal celebrates after scoring his team’s second goal during the Premier League match between Arsenal FC and Burnley FC at Emirates Stadium on December 22, 2018 in London, United Kingdom. (Photo by Shaun Botterill/Getty Images)Meanwhile, Aubameyang has had a sensational start to life at Arsenal following his club-record £56m arrival from Borussia Dortmund in January.Crouch: Liverpool could beat Man United to Jadon Sancho Andrew Smyth – September 14, 2019 Peter Crouch wouldn’t be surprised to see Jadon Sancho end up at Liverpool one day instead of his long-term pursuers Manchester United.The Gabon striker, who won the CAF Player of the Year in 2015, is level with Salah and Harry Kane as the league’s top scorer on 13 goals.The CAF announced on Twitter Aubameyang, Salah and Mane as finalists for the 2018 Player of the Year award as a result of their achievements over the past 12 months.The winner will be announced in a ceremony in Dakar, Senegal, next week.The Final 3 nominees for the African Player of the Year 2018 has been revealed, Who will be the King of Africa? @Aubameyang7 @10SadioMane @MoSalah #Final3 #AiteoCAFAwards18 pic.twitter.com/uAZ5BilxKA— CAF (@CAF_Online) January 1, 2019
Virgil van Dijk feels Liverpool have the quality to achieve great things on the pitch and further suggested he aims to be a club legend.The Dutch skipper has racked up 54 appearances and two goals for the Reds since he joined from Southampton in January 2018.Van Dijk has helped stabilise the Reds defence with the club boasting the best defensive record with 14 clean sheets and the least number of goals conceded so far this season.Asked by BBC’s Football Daily podcast how he’d like to be remembered by those fans in the future, Van Dijk replied: “That’s a difficult question, but maybe as a legend of Liverpool.“At the moment, that’s something that comes to my mind. I want to achieve great things, amazing things, here. We have a fantastic squad, we have everything, we have all the tools, but obviously, we have to do it.“For us, we have a team in the league that is pretty good as well [Manchester City] that are doing fantastic. We’re matching them at the moment and we will see what happens.“I just want to give everything for this club. They went all out to get me and I want to give everything for them and hopefully make sure we achieve great things.Van Dijk isn’t better than Messi & Ronaldo, says Van der Vaart Andrew Smyth – September 12, 2019 Rafael van der Vaart reckons Virgil van Dijk is “special”, but he’s still behind Lionel Messi and Cristiano Ronaldo as the world’s best players.“Before I started training [with the club], I went to the game against Leicester [in December 2017]. I was in the boardroom and met so many legends, great players who played for a beautiful club.”Before I signed my contract, I was 15 or 16 and working as a dishwasher in a Breda restaurant.”An in-depth interview on the life of Virgil van Dijk: https://t.co/csiJ7VE1Fx pic.twitter.com/aWJ3PvRG0P— BBC Sport (@BBCSport) February 21, 2019“When you play for this club, you’ll always be welcome as you’ve been part of the family. It’s one of the reasons I definitely wanted to play for the club.”The Reds will send out a message to Man City if they beat arch-rivals Manchester United to secure the top spot on Sunday.
Matchup History PDF Box Score LOUISVILLE, Ky. (AP) — Jordan Nwora scored 22 points and Louisville had to fend off Lipscomb in the closing minute for a 72-68 victory Wednesday night.Malik Williams added 10 points and 13 rebounds for Louisville (7-3), which rebounded from a 68-67 loss at Indiana on Saturday that snapped a three-game win streak.Garrison Matthews led Lipscomb (7-3) with five 3-pointers and 20 points. Rob Marberry added 11 points.Matthews made four free throws to pull the Bisons to 66-65 with 1:32 remaining. Williams then split a pair of free throws and Darius Perry forced a Lipscomb turnover. Following a Louisville timeout, Christen Cunningham’s jumper stretched the Cardinals’ lead to 69-65 with 21 seconds left. Another Lipscomb turnover and two free throws from Ryan McMahon made it 71-65 with 10 seconds left.Nwora ignited the Cardinals, scoring their first 11 points. His 3-pointer and dunk pushed the Louisville advantage to 29-21 with 5:53 left in the half, but Lipscomb rallied to 34-33 when Nathan Moran found Matt Rose for a 3-pointer at the buzzer.Lipscomb grabbed its first lead at 35-34 on a bucket by Michael Buckland to open the second half. Louisville pushed its lead back to 12, but Lipscomb tied it at 52 with 9:25 to go.BIG PICTURELouisville: The Cardinals figured to have an easier time following a tough loss to Indiana, but found themselves in another battle. Williams was a force on the boards grabbing three offensive and 10 defensive rebounds as the Cardinals outrebounded the Bisons 49-35. The Cardinals shot just 23 of 61 (38 percent) from the field.Lipscomb: The Bisons entered averaging 85.6 points after beating Navy 107-81 on Saturday and losing to Nashville neighbor Belmont 76-74 on Dec. 4. Despite struggling to get shots to fall against Louisville- shooting 23 of 65-they still had a chance late against the Cardinals.UP NEXTLipscomb hosts Division III Covent on Monday night. The Bisons return home after playing six of their last seven on the road.Louisville hosts Kent State on Saturday as part of a five-game homestand that includes No. 19 Kentucky on Dec. 29. Malik Williams Postgame Preview Buy Tickets Live Stats Listen Live Watch Live Full Schedule Roster Photo Gallery Dwayne Sutton Postgame Story Links Next Game: KENT STATE 12/15/2018 | 4 PM Print Friendly Version Postgame Quotes Postgame Notes Chris Mack Postgame Press Conference Regional Sports Networks
Controlled nucleation is essential for robust self-assembly. (A) An example 86-strand DNA-brick structure and (B) its associated connectivity graph. (C) Incidental interactions between dangling ends, shown in orange, lead to incorrect associations between fragments. Credit: (c) 2015 PNAS, DOI: 10.1073/pnas.1502210112 This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only. (Phys.org)—DNA bricks are an odd phenomenon. They are nanostructures built from synthetic, single-strand DNA that self-assemble into 3D structures. Several years ago, researchers at Wyss Institute at Harvard demonstrated the self-assembly of designed 3D structures made from 1,000 “bricks” that fit together like molecular Legos. One of the curiosities about these DNA bricks is why this complex self-assembly process works so well. Journal information: Proceedings of the National Academy of Sciences In an effort to uncover the nuances of DNA brick self-assembly, William M. Jacobs, Aleks Reinhardt, and Daan Frenkel from the University of Cambridge conducted theoretical studies to understand what drives the assembly process. Their studies reveal how temperature, kinetics, coordination number, and bond energies, contribute to the unique behavior of DNA brick self-assembly, and while it is a nucleated process, it does not behave according to classical nucleation theory. Their work appears in the Proceedings of the National Academy of Sciences.In order to optimize DNA brick assembly, Jacobs, et al. wanted to investigate whether these systems had a nucleation barrier. In classic nucleation theory (CNT), the nucleation barrier is the energy cost for making an ordered structure, such as a crystal. This energy barrier must be overcome before a compound can settle into its equilibrium structure. The authors found that DNA brick assembly does have a nucleation barrier, but, unlike CNT, this barrier is determined by the way the bricks are connected in the target structure. They identified several factors that contribute to this non-classical behavior.DNA bricks are designed to assemble in particular ways by matching certain sections of a DNA strand with a complimentary section. These designed interactions need to be stronger than incidental interactions. They found that there is a critical number of DNA strands that must be assembled to promote the formation of the target structure, meaning that the the nucleation barrier is a consequence of the number of subunits that must be assembled.Additionally, DNA-bricks have a certain temperature range at which the nucleation barrier is overcome, but over which the free energy of the final structure is higher than the initial monomers. This deviates from CNT which predicts that a nucleus with more than the critical number of DNA strands will assemble into the complete structure. This difference is due to DNA bricks forming stable intermediate structures. Experimentally, this means that self-assembly occurs in a narrow temperature range in which nucleation begins at a high temperature, and as the structure is gradually cooled, the target structure is favored.In prior studies, the maximum number of bonds made by each brick in the interior of a structure was four, but it is possible to have different coordination numbers. The question was whether the coordination number affected the nucleation barrier, and whether four bonds were necessary. It turns out that DNA brick self-assembly works well because each subunit makes only a small number of bonds. When the authors tested higher coordination numbers, they found that the system behaved analogously to CNT.Finally, Jacobs et al. found that contrary to theories that homogenous bond energies would decrease the number of errors during DNA brick assembly, it is the diversity in bond energies that contributes to enhanced kinetics of DNA brick self-assembly. This was likely due to stabilizing unstable intermediate structures.Jacobs, et al. have identified several key features that aid in understanding why DNA brick self-assembly works as well as it does. Features such as ramping down the temperature and optimizing the coordination number explain why there are so few errors in the self-assembly process. And, their discovery that heterogeneous bond energies promote target molecule formation is a practical place where researchers can improve yields and decrease errors in DNA brick assembly. More information: “Rational design of self-assembly pathways for complex multicomponent structures” PNAS, DOI: 10.1073/pnas.1502210112AbstractThe field of complex self-assembly is moving toward the design of multiparticle structures consisting of thousands of distinct building blocks. To exploit the potential benefits of structures with such “addressable complexity,” we need to understand the factors that optimize the yield and the kinetics of self-assembly. Here we use a simple theoretical method to explain the key features responsible for the unexpected success of DNA-brick experiments, which are currently the only demonstration of reliable self-assembly with such a large number of components. Simulations confirm that our theory accurately predicts the narrow temperature window in which error-free assembly can occur. Even more strikingly, our theory predicts that correct assembly of the complete structure may require a time-dependent experimental protocol. Furthermore, we predict that low coordination numbers result in nonclassical nucleation behavior, which we find to be essential for achieving optimal nucleation kinetics under mild growth conditions. We also show that, rather surprisingly, the use of heterogeneous bond energies improves the nucleation kinetics and in fact appears to be necessary for assembling certain intricate 3D structures. This observation makes it possible to sculpt nucleation pathways by tuning the distribution of interaction strengths. These insights not only suggest how to improve the design of structures based on DNA bricks, but also point the way toward the creation of a much wider class of chemical or colloidal structures with addressable complexity.
In March tourist arrivals to the Northern Marianas continue to rise, up five percent to 42,539 visitors, with 40,613 visitors during the same period last year. According to the Marianas Visitors Authority (MVA), arrivals to the islands of Saipan, Tinian and Rota registered 42,539 visitors in March 2015, compared to 40,613 visitors received in March 2014.Arrivals from Korea soared 40% to 14,989 visitors. Jeju Air launched an additional daily flight to Saipan from Incheon beginning in late March, ensuring extremely rapid growth in the Korean arrivals for the remainder of this year.Arrivals from China also increased 31% in March to 16,246 visitors. The MVA also launched a variety of outdoor advertising campaigns in Shanghai, Guangzhou and Chengdu in support of China charter flights.